Overlay histogram showing Ntera-2 cells stained with CSB-MA018403A0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4-¦C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4-¦C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot
Positive WB detected in: Mouse brain tissue, Rat brain tissue
All lanes: OCT4 antibody at 1:1000, 1:5000, 1:8000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 39, 31 kDa
Observed band size: 45 kDa

IHC image of CSB-MA018403A0m diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4-¦C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA018403A0m diluted at 1:100 and staining in paraffin-embedded human-átestis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4-¦C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.